AFTER PCR SEQUENCING FULL LENGTH
See the edits in your PCR results.
CRISPR Profiling analyzes usable full-length PCR sequencing results already in your account. Compare edited samples with a wild-type reference, inspect cut-site alleles, and download the result package. This is analysis of a completed PCR order, not a second sample submission.
Complete PCR sequencing
Submit a PCR Sequencing Full Length order with a wild-type reference and edited samples. Wait until usable consensus outputs are available.
Choose your comparison
Sign in, select the completed order, choose the reference and edited samples, then use automatic cut-site detection or enter the cut site manually.
Review your profile
Inspect editing metrics and cut-site alleles, then download the complete result package.
01 / WHAT YOU NEED
Start with a PCR Full Length order.
The analysis uses passed consensus outputs from the PCR Full Length pipeline. You need a wild-type reference and at least one edited sample from the eligible completed order. An order without usable outputs cannot start an analysis.
02 / ANALYZE
Choose how to locate the cut site.
Compare edited reads against the wild-type reference to detect a cut site automatically, or enter a cut position manually. The analysis keeps the selected reference, samples, and cut-site settings with the result.
03 / WHAT YOU RECEIVE
Editing metrics and allele detail.
- Per-sample raw and wild-type-background-adjusted indel rates.
- Counts of edited, no-indel, and informative reads.
- Cut-site allele details and available sequence alignments.
- A downloadable result package for your records.
Results depend on the quality and coverage of the completed PCR outputs.
READY WHEN YOUR PCR RESULTS ARE
Profile your edited samples.
Sign in to analyze an eligible completed PCR Full Length order.