Texas family owned · Sugar Land, TXFROM PCR MACHINE TO GENOTYPE
Tissue and Cell Line Genotyping, Made Simple.
Straight from your PCR machine to a genotype report. Run your PCR, ship the products as they are, and get genotypes back — no more running gels, no more cutting bands, no more PCR product clean-up, no Sanger primer mix. Every read is matched against the exact sequences that tell your alleles apart: primers, product size, or an internal hot spot.
Pick or build an assay
Start from a prebuilt JAX assay, or paste your amplicon and mark the regions that matter.
Ship your PCR products
As they are — same intake as our PCR sequencing service. One plate or tube set, one order.
Sequence, match, report
Full-length nanopore reads are matched against your assay; you receive per-sample, per-target read counts with QC.
01 / JAX ASSAYS
Working with JAX mice?
We can convert any assay protocol from the JAX genotyping protocol database into a ready-to-run assay — just tell us the stock number or protocol. Prebuilt JAX assays are already available in your assay list. JAX recommends independent validation of assays on receipt of animals; we can verify any assay against your real reads.
Genotyping
Assay-based genotyping from full-length PCR reads — no gels, sequence-verified calls.
$7.00 / sample
Sample guidelines (PDF) ↗02 / YOUR OWN ASSAY
Or build your own.
Paste the amplicon you expect and highlight what must match exactly: the primers at each end and any hot spot that separates your alleles. Spacing is measured for you, so two same-length alleles can still be told apart by sequence. Assays are versioned, and approved versions are frozen into each order so your report always states exactly what it was matched against. Our team reviews every assay and sets QC thresholds before it can be ordered.
03 / SEE THE DIFFERENCE
No more running gels, no more cutting bands.
The same three mice, both ways. A gel gives you band positions to interpret by eye; the report gives you counted, sequence-verified reads per allele — including small indels a gel cannot resolve.
| Sample | Reads | Mutant (217 bp) | Wild type (179 bp) | Genotype | QC |
|---|---|---|---|---|---|
| mouse_01 | 1,846 | 903 (48.9%) | 943 (51.1%) | Heterozygote | PASS |
| mouse_02 | 2,110 | 7 (0.3%) | 2,103 (99.7%) | Wild type | PASS |
| mouse_03 | 1,592 | 1,581 (99.3%) | 11 (0.7%) | Homozygous mutant | PASS |
The case neither method can call: a small indel
A heterozygous 7 bp deletion. On a gel the 217 and 210 bp products co-migrate as one band. Sanger sequencing of the same animal reads cleanly up to the indel — then the two alleles fall out of frame and every position becomes a mixed double peak. Per-read sequence matching has no mixture to untangle: each read carries one allele, so both are simply counted.
| Sample | Reads | WT (217 bp) | Δ7 indel (210 bp) | Genotype |
|---|---|---|---|---|
| het_01 | 1,933 | 968 (50.1%) | 965 (49.9%) | Heterozygote |
04 / WHAT YOU RECEIVE
Counted, verified, reported.
- Genotyping report (PDF) — per-sample, per-target read counts with percentages, QC outcome, and genotype calls where the assay defines rules (for example Homozygous / Heterozygote / Wild type).
- Evidence files — the same results as a spreadsheet-ready TSV and a browsable HTML report, plus the assay checksum for traceability.
- Sequencing evidence — consensus sequences, per-sample reports and gel-style summaries from the underlying full-length PCR run.
- Raw reads (optional) — the debarcoded FASTQ behind every call.
05 / GOOD TO KNOW
Conservative by design.
Matching is exact: a read with errors in a diagnostic region stays unassigned rather than becoming a confident call.
READY WHEN YOU ARE
Ready to genotype without gels?
Sign in or create your account to get started.
Ask us about your assay
LET’S TALK
Questions about an assay? Send them here.