Texas family owned · Sugar Land, TX

OXFORD NANOPORE · FROM PCR MACHINE TO FULL-LENGTH SEQUENCE S (a little mouse is pushing it into place)

Multiple Amplicon Sequencing in One Tube, Made Simple.

Ship your PCR products as they come off the machine — unpurified, no sequencing primer, any linear product over 100 bp. Every amplicon is read end to end in a single nanopore read, so you get the complete sequence in one pass. Put several amplicons in one tube and each is sequenced and counted separately: ideal for both sequencing and quantification of mixed products.

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Overnight after arrivalUnpurified PCR acceptedNo sequencing primerMultiple amplicons per tubeFASTA + AB1 deliveredCluster counts for quantification

STEP 01

Run your PCR

Any protocol, any polymerase. Products over 100 bp; several amplicons in one tube are welcome when their lengths differ by at least 10%.

STEP 02

Ship it as it is

No clean-up, no primer mix. Drop samples at a nearby Dropbox or ship them to the lab.

STEP 03

Sequence, cluster, report

Reads are clustered by amplicon and each cluster gets its own consensus. Results land in your account overnight after arrival.

01 / MULTIPLE AMPLICONS, ONE TUBE

Sequence and quantify mixed products in a single reaction.

A gel tells you how many bands you have and roughly how big they are. Full-length nanopore reads tell you what each one is. Reads from one tube are clustered by amplicon, every cluster is assembled into its own consensus, and the read count per cluster reports the relative abundance of each product — so one submission gives you both the sequences and the ratio.

Simulated agarose gel: ladder plus one sample lane with three bands at 690, 458 and 312 bp — What you used to run: three bands, sizes only.

The same tube sequenced: three amplicon clusters with read counts and percentages — What you receive instead: each amplicon sequenced separately, with counts.

Cluster	Length	Reads	Share	Delivered
Amplicon A	312 bp	1,240	62.0%	FASTA · AB1 · per-base breakdown
Amplicon B	458 bp	620	31.0%	FASTA · AB1 · per-base breakdown
Amplicon C	690 bp	140	7.0%	FASTA · AB1 · per-base breakdown

Illustrative example. Clusters are separated when amplicon lengths differ by at least 10%.

02 / BEYOND SANGER PEAKS

One molecule per read — no mixed peaks to untangle.

Sanger sequencing averages every molecule in the tube into a single trace. Two products, a heterozygous indel, or a secondary band all collapse into overlapping double peaks that no base caller can read. This service runs on the same production line as our genotyping service: each nanopore read is a single molecule carrying a single sequence, so mixtures separate cleanly into their own consensus sequences instead of blurring into one. Non-specific products are filtered out during data processing rather than spoiling the read.

Simulated Sanger chromatogram: clean peaks that turn into unreadable mixed double peaks after an indel — Sanger on a mixed sample: unreadable double peaks after the point where the products diverge.

Sample	Reads	Product 1 (217 bp)	Product 2 (210 bp)
mix_01	1,933	968 (50.1%)	965 (49.9%)

This service: both products are read whole and counted — the 7 bp difference is resolved from sequence.

03 / PRICING

Standard public prices, per sample.

PCR Sequencing Full Length from $5.00 per sample ($4.50 from 9 samples in an order). Optional raw read delivery (FASTQ): $0.25 per sample, maximum $10 per order. Get an instant quote on our public quotation page — no login required.

PCR

PCR Sequencing Full Length

Linear PCR product over 100 bp. Multiple amplicons can be sequenced in one tube, with a separate sequence returned for each amplicon, provided their lengths differ by at least 10%.

$5.00 / sample

Sample guidelines (PDF) ↗

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04 / WHAT YOU RECEIVE

Sequence, trace, breakdown, counts.

FASTA — the full-length consensus sequence of every amplicon cluster in the tube.
AB1 trace — a Sanger-style trace file per amplicon that opens in the chromatogram viewer you already use.
Per-base breakdown — position-by-position base calls with read support, so you can see exactly how well every base is backed.
Cluster counts — reads per amplicon and their percentages: the quantification behind mixed-product submissions.
Raw reads (optional) — the debarcoded FASTQ behind every consensus.

05 / SAMPLE REQUIREMENTS

What to send.

Linear PCR product over 100 bp.
Unpurified is fine — straight from the machine, at no extra charge. No sequencing primer.
Several amplicons in one tube are sequenced and counted separately when their lengths differ by at least 10%.
Same intake as our genotyping service: one plate or tube set, one order. Products over 1 kb: tell us before ordering.

06 / GOOD TO KNOW

Sequenced in Texas, in your account.

Texas family owned, with sequencing performed in Texas. Orders, billing and results live in one secure account, with purchase orders and lab sharing supported.

Four free samples for new customersInstant quote without a loginDropbox sites or courier intakeOvernight turnaround after arrival in lab

READY WHEN YOU ARE

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