PCR Sequencing · CVENTBIO
Full-Length PCR Sequencing
Send unpurified PCR products without a sequencing primer. Receive consensus sequences, read counts, and reports for supported amplicon clusters.
Run your PCR
Any protocol, any polymerase. Products over 100 bp; several amplicons in one tube are welcome when their lengths differ by at least 10%.
Ship it as it is
No clean-up, no primer mix. Drop samples at a nearby Dropbox or ship them to the lab.
Sequence, cluster, report
Reads are clustered by amplicon and each cluster gets its own consensus. Results land in your account overnight after arrival.
01 / MULTIPLE AMPLICONS, ONE TUBE
Sequence and quantify mixed products in a single reaction.
A gel tells you how many bands you have and roughly how big they are. Full-length nanopore reads tell you what each one is. Reads from one tube are clustered by amplicon, every cluster is assembled into its own consensus, and the read count per cluster reports the relative abundance of each product — so one submission gives you both the sequences and the ratio.
| Cluster | Length | Reads | Share | Delivered |
|---|---|---|---|---|
| Amplicon A | 312 bp | 1,240 | 62.0% | FASTA · AB1 · per-base breakdown |
| Amplicon B | 458 bp | 620 | 31.0% | FASTA · AB1 · per-base breakdown |
| Amplicon C | 690 bp | 140 | 7.0% | FASTA · AB1 · per-base breakdown |
Looking for small sequence differences?
Routine amplicon clustering and target-specific allele analysis are different workflows. The size-based examples above do not establish single-base or small-indel detection.
For near-identical variants, use experimental Allele Analysis on retained reads, or prepare a reviewed genotyping assay. Suitability depends on the target, read quality, and evidence.
Pricing
Standard per-sample pricing is shown below. Use the quote calculator for quantity pricing and optional raw-read delivery.
PCR Sequencing Full Length
PCR product 100 bp - 5 kb. Multiple amplicons can be sequenced in one tube. Regular PCR, Allele Analysis, and Genotyping PCR choose this service.
$5.00 / sample
Sample guidelines (PDF) ↗04 / WHAT YOU RECEIVE
Sequence, trace, breakdown, counts.
- FASTA — the full-length consensus sequence of every amplicon cluster in the tube.
- AB1 trace — a Sanger-style trace file per amplicon that opens in the chromatogram viewer you already use.
- Per-base breakdown — position-by-position base calls with read support, so you can see exactly how well every base is backed.
- Cluster counts — reads per amplicon and their percentages: the quantification behind mixed-product submissions.
- Raw reads (optional) — the debarcoded FASTQ behind every consensus.
AFTER SEQUENCING / CRISPR PROFILING
Turn completed PCR results into an edit profile.
If your PCR Sequencing Full Length order includes a wild-type reference and edited samples with usable results, you can profile cut-site edits from those existing reads. No second sample submission is needed.
05 / SAMPLE REQUIREMENTS
What to send.
- Linear PCR product over 100 bp.
- Unpurified is fine — straight from the machine, at no extra charge. No sequencing primer.
- Several amplicons in one tube are sequenced and counted separately when their lengths differ by at least 10%.
- Same intake as our genotyping service: one plate or tube set, one order. Products over 1 kb: tell us before ordering.
06 / GOOD TO KNOW
Sequenced in Texas, in your account.
Texas family owned, with sequencing performed in Texas. Orders, billing and results live in one secure account, with purchase orders and lab sharing supported.
READY WHEN YOU ARE
Ready to sequence your PCR products?
Continue in your account to get started.
Ask us about your amplicons
LET’S TALK
Questions about your amplicons or a mixed product? Send them here.