Allele Analysis · CVENTBIO

Discover & Compare Alleles

Analyze retained reads from a completed PCR order. Define your target regions, discover supported variants, or search for expected allele sequences.

Experimental analysis. Detection limits and biological accuracy remain unvalidated. Results depend on read quality and coverage.

From strands to alleles

Conceptual illustrations; example sequences and percentages.

See distinct, supported alleles when a mixed Sanger trace is difficult to interpret.

Compare substitutions, insertions and deletions against your reference.

GC-rich amplicons can be analyzed with sufficient amplification and usable read coverage.

See each supported allele’s sequence, length, reference differences, supporting reads and read percentage.

Allele	Length	Support / usable reads	Reads %
A1	240 bp	700 / 1,000	70%
A2	237 bp	200 / 1,000	20%
A3	240 bp	80 / 1,000	8%

2% of usable reads are unassigned in this example. Percentages describe usable reads.

Download your results:Summary CSVTarget FASTAFull-amplicon FASTA, when supportedAnalysis manifest

Identify supported sequence variants between your chosen flanks and compare them with a reference.

Search for up to four expected allele sequences. Results show detected, not detected or inconclusive when evidence is insufficient.

Select your order and samples. Use completed full-length PCR sequencing results with retained reads.
Define your target. Enter shared left and right flanks (15–200 bases each) and a reference target (1–1,000 bases).
Run and review. Choose an analysis mode, then view your allele report and download the data.

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Sugar Land, TX 77479

info@cventbioseq.com

Single-molecule allele analysis